欧州骨粗鬆症学会 in bordeaux 2012

bordeauxタイトル

第12回欧州骨粗鬆症学会(2012/3/21〜24)に 参加しました。

IOF-ECCE012ガイドブック
以下はシンポジウムで発表した概要です。

Effects of Rosemary Carnosic Acid on Bone Metabosism

Hiromi Hagiwara1, Roshan Basnet1 and Keitaro Hagiwara2

1 Department of Biomedical Engineering, Toin University of Yokohama. Yokohama, Kanagawa 225-8503, Japan,
2 Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8501, Japan


In bone tissues, both the formation and maintenance of bone are controlled by bone-forming osteoblasts and bone-resorbing osteoclasts, and an imbalance between these two types of cell leads to the bone metabolic diseases, such as osteoporosis and osteopetrosis. The formation of bone involves a complex series of events that include the proliferation and differentiation of osteoprogenitor cells and eventually result in the formation of a mineralized extracellular matrix. We used preosteoblastic cell MC3T3-E1. On the other hand, osteoclasts are multinucleated giant cells with the ability to resorb mineralized tissues. They are formed from haematopoietic cells of the monocyte/macrophage lineage. The formation and activation of osteoclasts are controlled by the combined action of the receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). In the present study, we used a culture of mouse spleen cells with stromal cell line ST2 had been treated with activated vitamin D3.
Polyphenols reportedly exert physiological effects against diseases such as cancer, arteriosclerosis, hyperlipidaemia and osteoporosis. We previously reported that quercetin from onion, curcumin from curcuma longa, and apigenin from pasley and olive inhibit the mineralization of osteoblastic cells and the formation of osteoclasts. Recently, we examined effects of olive polyphenols such as oleuropein, hydroxytyrosol, and tyrosol on bone formation using cultured osteoblasts and osteoclasts, and on bone loss in ovariectomized mice and we showed that oleuropein and hydroxytyrosol enhanced bone formation in vivo and in vitro.
In the present study, we designed to clarify the effects of rosemary carnosic acid on bone metabolism using cultured osteoblasts and osteoclasts. Carnosic acid inhibited the proliferation of preosteoblastic MC3T3-E1 cells. Furthermore, carnosic acid decreased in the activity of alkaline phosphatase and the deposition of calcium in a dose-dependent manner. On the other hand, this compound also inhibited the formation of multinucleated osteoclasts in a dose-dependent manner. Our findings indicate that carnosic acid may have critical effects on the formation and maintenance of bone.

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