欧州硬組織学会 in Salzburg 2017
the 44th European Calcified Tissue Society Congressに
参加しました。
以下はシンポジウムで発表した概要です。
ECTS 2017 13-15 May, Salzburg, AUSTRIA
4-Hydroxyderricin inhibits osteoclast formation with attenuation of expression of RANKL mRNA
Faculty of Biomedical Engineering, Toin University of Yokohama, 1614 Kurogane-cho, Aoba-ku, Yokohama 225-8503, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
Abstract
4-Hydroxyderricin (4-HD) is a major polyphenol of Angelica keiskei (Ashitaba), exhibiting antiallergic, antidiabetic, antioxidant, and antitumor effects. The present study was designed to evaluate the effects of 4-HD on osteoclast formation by using cultured osteoclasts. 4-HD did not affect cell proliferation of stromal ST2 cells at concentrations of 1 μM to 10 μM. This compound inhibited the formation of multinucleated osteoclasts from mouse splenic cells, and we identified a molecular pathway of osteoclast differentiation mediated by 4-HD, which led to inhibition of the expression of receptor activator of nuclear factor-κB ligand and macrophage-colony stimulating factor in ST2 cells. Furthermore, 4-HD decreased H2O2 levels in ST2 cells at 1 μM and 3 μM, but increased at 10 μM. Our findings indicate that 4-HD may have critical effects on bone formation.
Keywords Chalcone・4-Hydroxyderricin・Polyphenol・Osteoclast・RANKL
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