欧州骨粗鬆症学会 in Seville 2014
2014欧州骨粗鬆症学会(2014/4/2～5)に
参加しました。
以下はシンポジウムで発表した概要です。
WCO-IFO-ESCEO SEVILLE 2014, April 2-5,2014, Seville, Spain
Flavonol Inhibits Formation of Osteoclasts in Culture System rather than Flavanone
Hiromi Hagiwara, Kyoko Nakata, Sundari Indah Wiyasihati, and Kaoru Yoshida
Department of Biomedical Engineering, Toin University of Yokohama,
1614 Kurogane-cho, Aoba-ku, Yokohama 225-8503, Japan
Tel: +81-45-974-5057, Fax: +81-45-972-5972, E-mail: hagiwara(at)cc.toin.ac.jp
Biomedical Engineering Center, Toin University of Yokohama,
1614 Kurogane-cho, Aoba-ku, Yokohama 225-8503, Japan
Department of Physiology, School of Medicine, Airlangga University, Surabaya, Indonesia
Objective: Polyphenol have been reported to have physiological effects with respect to alleviating diseases such as osteoporosis and osteopetrosis. We recently reported that the olive polyphenol hydroxytyrosol accelerates bone formation both in vivo and in vitro (Eur. J. Pharmacol. 2011, 662: 78-84). The present study was designed to evaluate the in vitro effects of flavonoid, such as flavonol and flavanone, on the formation of multinucleated osteoclasts in culture system. Materials and Methods: Multinucleated osteoclasts were formed from the mouse splenic cells by coculture system with ST2 cells stimulated by 100 nM vitamin D3. Osteoclastic cells were fixed and stained for TRAP activity. TRAP-positive multinucleated cells (five or more nuclei) were counted under a microscope. We used fisetin, quercetin, and rhamnetin as flavonol and hesperetin, naringenin, and sakuranetin as flavanone in this study. Results: Fisetin, quercetin, and rhamnetin at 10 μM completely inhibited the formation of multinucleated osteoclasts. By contrast, the weak inhibition of osteoclast formation were observed with hesperetin, naringenin, and sakuranetin at 10 μM. Conclusion: These results suggested that the hydroxyl group at carbon 3-position of flavonol might play a critical role on inhibition of osteoclast formation.
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